Análise genética de estirpes de Schistosoma mansoni sensíveis e resistentes a praziquantel por rapd-pcr
Palavras-chave:
Schistosoma mansoni, RAPD-PCR, resistência ao Praziquantel
Resumo
O praziquantel (PZQ) é o fármaco de eleição no tratamento da schistosomose, devido à sua alta taxa de cura e por não demostrar efeitos secundários significativos mas recentemente têm sido reportados cada vez mais casos de resistência ou de aumento de tolerância a este fármaco. Devido a estas observações, a OMS recomenda a monitorização contínua de Schistosoma spp potencialmente resistentes ao fármaco. No laboratório de Helmintologia foi seleccionada uma linha de Schistosoma mansoni, resistente a PZQ na dose de 120 mg/Kg, após pressão de fármaco constante ao longo de vários ciclos. Foram recolhidos parasitas adultos de S. mansoni da estirpe resistente e de uma estirpe sensível e procedeu-se à análise do DNA dos vermes por Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). Foi possível observar polimorfismos em 7 dos 10 primers. Uma vez observadas as diferenças polimórficas entre parasitas sensíveis e resistentes, calculou-se o coeficiente de similaridade (Dice’s coefficient). A compreensão de polimorfismos genéticos associados à resistência poderão contribuir para a identificação futura de sequências relacionadas com a resistência/tolerância de S. mansoni ao tratamento por PZQ e para a identificação de parasitas resistentes no campo, assim como para o desenvolvimento de novas estratégias de controlo para a schistosomose.
Downloads
Não há dados estatísticos.
Referências
1. Xiao Sh., Utzinger, J., Chollet, J., & Tanner, M. (2006). Effect Of Artemether
Administered Alone Or In Combination With Praziquantel To Mice Infected With
Plasmodium berghei Or Schistosoma mansoni Or Both. International Journal For Parasitology
, 36: 957-964
2. Reimert, C. M., Tukahebwa, E. M., Kabatereine, N. B., Dunnec, D. W., & Vennervald,
B. J. (2008). Assessment Of Schistosoma mansoni Induced Intestinal Inflammation
By Means Of Eosinophil Cationic Protein, Eosinophil Protein X And Myeloperoxidase
Before And After Treatment With Praziquantel. Acta Tropica , 105, 253-259.
3. Aly, I. R., Hendawy, M. A., Ali, E., Hassan, E., & Nosseir, M. M. (2010). Immunological
And Parasitological Parameters After Treatment With Dexamethasone In
Murine Schistosoma mansoni. Mem Inst Oswaldo Cruz , 105(6): 729-735.
4. Ernould, J.-C., Ba, K., & Sellin, B. (1999). Increase Of Intestinal Schistosomiasis
After Praziquantel Treatment In A Schistosoma haematobium And Schistosoma mansoni
Mixed Focus. Acta Tropica , 73: 143-152
5. Pica-Mattoccia, L., & Donato, C. (2004). Sex- And Stage-Related Sensitivity
Of Schistosoma mansoni To In Vivo And In Vitro Praziquantel Treatment. International
Journal For Parasitology , 34: 527-533.
6. Raso, G., N’goran, E. K., Toty, A., Luginbühl, A., Adjoua, C. A., Tian-Bi, N. T.,
Et Al. (2004). Efficacy And Side Effects Of Praziquantel Against Schistosoma mansoni
In A Community Of Western Côte D’ivoire. Transactions Of The Royal Society Of
Tropical Medicine And Hygiene , 98: 18-27.
7. Hines-Kay J, Cupit P, Sanchez M, Rosenberg G, Hanelt B, Cunningham C (2012).
Transcriptional Analysis Of Schistosoma mansoni Treated With Praziquantel In Vitro.
Molecular & Biochemical Parasitology 186: 87– 94
8. Pica-Mattoccia L, Doenhoff M, Valle C, Basso A, Troiani Ar, Liberti P, Festucci A,
Guidi A, Cioli D (2009). Genetic Analysis Of Decreased Praziquantel Sensitivity In A
Laboratory Strain Of Schistosoma mansoni . Acta Tropica 111: 82–85
9. Wang W, Wang L, Liang Ys (2012).Susceptibility Or Resistance Of Praziquantel In
Human Schistosomiasis: A Review. Parasitol Res 111: 1871–1877
10. Couto F, Coelho P, Araújo N, Kusel J, Katz N, Jannotti-Passos L, Mattos A (2011).
Schistosoma mansoni: A Method For Inducing Resistance To Praziquantel Using Infected
Biomphalaria glabrata Snails. Mem Inst Oswaldo Cruz 106: 153-157
11. Doenhoff, M. J., Kusel, J. R., Coles, G. C., & Cioli, D. (2002). Resistence Of
Schistosoma mansoni To Praziquantel: Is There A Problem? Transactions Of The Royal
Society Of Tropical Medicine And Hygiene , 96: 465-469.
12. Ferrari, T. C., Moreira, P. R., & Cunha, A. S. (2008). Clinical Characterization
Of Neuroshistosomiasis Due To Schistosoma mansoni And Its Treatment. Acta Tropica,
108: 89-97.
13. Lamberton, P. H., Hogan, S. C., Kabatereine, N. B., Fenwick, A., & Webter, J. P.
(2010). In Vitro Praziquantel Test Capable Of Detecting Reduced In Vivo Efficacy In
Schistosoma mansoni Human Infections. American Journal Of Tropical Medicine And
Hygiene , 83: 1340-1347
14. Midzi, N., Sangweme, D., Zinyowera, S., Mapingure, M., Brouwer, K., Kumar,
N., Et Al. (2008). Efficacy And Side Effects Of Praziquantel Treatment Against Schistosoma
haematobium Infection Among Primary School Children In Zimbabwe. Transactions
Of The Royal Society Of Tropical Medicine And Hygiene , 102: 759-766.
15. Dajem S, Ibrahim E, Al-Quraishy S, Mostafa O (2011). Fingerprint Of Biomphalaria
arabica, The Intermediate Host Of Schistosoma mansoni In Saudi Arabia, Using Rapd-
-Pcr. Gene 485: 69–72
16. Shalaby L., Gherbawy, Y, Banaja A (2011). Genetic Diversity Among Schistosoma
mansoni Population In The Western Region Of Saudi Arabia. Tropical Biomedicine 28:
90–101
17. Sire C, Durand P, Pointier J-P, Theron A (2001). Genetic Diversity Of Schistosoma
mansoni Within And Among Individual Hosts (Rattus Rattus): Infrapopulation
Differentiation At Microspatial Scale. International Journal For Parasitology 31:
1609–1616
18. Stothard, J. R., Hughes, S., & Rollinson, D. (1996). Variation Within The Internal
Transcribed Spacer (Its) Of Ribosomal Dna Genes Of Intermediate Snail Hosts Within
The Genus Bulinus (Gastropoda: Planorbidae). Acta Tropica, 61(1):19-29
19. Tsai, M.-H., Marx, K. A., Ismail, M. M., & Tao, L.-F. (2000). Randomly Amplified
Polymorphic Dna (Rapd) Polymerase Chain Reaction Assay For Identification Of
Schistosoma mansoni Strains Sensitive Or Tolerant To Anti-Schistosomal Drugs. Journal
Of Parasitology, 86(1): 146-149.
20. Oliveira A, Silva D, Zanotti-Magalhaes E, Abdel-Hamid A, Ribeiro-Paes J (2008).
Schistosome/Mollusk: Genetic Compatibility. Genet. Mol. Res. 7: 518-526
21. Spada Rgm, Silva D, Abdel-Hamid A-Z , Sobral-Hamaguchi Ss, Zuim Nrb, Zanotti-
Magalhães Em, Magalhães La, Ribeiro-Paes Jt (2002). Genetic Markers Between
Biomphalaria glabrata Snails Susceptible And Resistant To Schistosoma mansoni Infection.
Mem Inst Oswaldo Cruz 97: 53-58
22. Barral, V., This P, Imbert-Establet D., Combes C., & Delseny M. (1993). Genetic
Variability And Evolution Of The Schistosoma Genome Analysed By Using Random
Amplified Polimorphic Dna Markers. Mol Biochem Parasitol., 59(2):211-21
Administered Alone Or In Combination With Praziquantel To Mice Infected With
Plasmodium berghei Or Schistosoma mansoni Or Both. International Journal For Parasitology
, 36: 957-964
2. Reimert, C. M., Tukahebwa, E. M., Kabatereine, N. B., Dunnec, D. W., & Vennervald,
B. J. (2008). Assessment Of Schistosoma mansoni Induced Intestinal Inflammation
By Means Of Eosinophil Cationic Protein, Eosinophil Protein X And Myeloperoxidase
Before And After Treatment With Praziquantel. Acta Tropica , 105, 253-259.
3. Aly, I. R., Hendawy, M. A., Ali, E., Hassan, E., & Nosseir, M. M. (2010). Immunological
And Parasitological Parameters After Treatment With Dexamethasone In
Murine Schistosoma mansoni. Mem Inst Oswaldo Cruz , 105(6): 729-735.
4. Ernould, J.-C., Ba, K., & Sellin, B. (1999). Increase Of Intestinal Schistosomiasis
After Praziquantel Treatment In A Schistosoma haematobium And Schistosoma mansoni
Mixed Focus. Acta Tropica , 73: 143-152
5. Pica-Mattoccia, L., & Donato, C. (2004). Sex- And Stage-Related Sensitivity
Of Schistosoma mansoni To In Vivo And In Vitro Praziquantel Treatment. International
Journal For Parasitology , 34: 527-533.
6. Raso, G., N’goran, E. K., Toty, A., Luginbühl, A., Adjoua, C. A., Tian-Bi, N. T.,
Et Al. (2004). Efficacy And Side Effects Of Praziquantel Against Schistosoma mansoni
In A Community Of Western Côte D’ivoire. Transactions Of The Royal Society Of
Tropical Medicine And Hygiene , 98: 18-27.
7. Hines-Kay J, Cupit P, Sanchez M, Rosenberg G, Hanelt B, Cunningham C (2012).
Transcriptional Analysis Of Schistosoma mansoni Treated With Praziquantel In Vitro.
Molecular & Biochemical Parasitology 186: 87– 94
8. Pica-Mattoccia L, Doenhoff M, Valle C, Basso A, Troiani Ar, Liberti P, Festucci A,
Guidi A, Cioli D (2009). Genetic Analysis Of Decreased Praziquantel Sensitivity In A
Laboratory Strain Of Schistosoma mansoni . Acta Tropica 111: 82–85
9. Wang W, Wang L, Liang Ys (2012).Susceptibility Or Resistance Of Praziquantel In
Human Schistosomiasis: A Review. Parasitol Res 111: 1871–1877
10. Couto F, Coelho P, Araújo N, Kusel J, Katz N, Jannotti-Passos L, Mattos A (2011).
Schistosoma mansoni: A Method For Inducing Resistance To Praziquantel Using Infected
Biomphalaria glabrata Snails. Mem Inst Oswaldo Cruz 106: 153-157
11. Doenhoff, M. J., Kusel, J. R., Coles, G. C., & Cioli, D. (2002). Resistence Of
Schistosoma mansoni To Praziquantel: Is There A Problem? Transactions Of The Royal
Society Of Tropical Medicine And Hygiene , 96: 465-469.
12. Ferrari, T. C., Moreira, P. R., & Cunha, A. S. (2008). Clinical Characterization
Of Neuroshistosomiasis Due To Schistosoma mansoni And Its Treatment. Acta Tropica,
108: 89-97.
13. Lamberton, P. H., Hogan, S. C., Kabatereine, N. B., Fenwick, A., & Webter, J. P.
(2010). In Vitro Praziquantel Test Capable Of Detecting Reduced In Vivo Efficacy In
Schistosoma mansoni Human Infections. American Journal Of Tropical Medicine And
Hygiene , 83: 1340-1347
14. Midzi, N., Sangweme, D., Zinyowera, S., Mapingure, M., Brouwer, K., Kumar,
N., Et Al. (2008). Efficacy And Side Effects Of Praziquantel Treatment Against Schistosoma
haematobium Infection Among Primary School Children In Zimbabwe. Transactions
Of The Royal Society Of Tropical Medicine And Hygiene , 102: 759-766.
15. Dajem S, Ibrahim E, Al-Quraishy S, Mostafa O (2011). Fingerprint Of Biomphalaria
arabica, The Intermediate Host Of Schistosoma mansoni In Saudi Arabia, Using Rapd-
-Pcr. Gene 485: 69–72
16. Shalaby L., Gherbawy, Y, Banaja A (2011). Genetic Diversity Among Schistosoma
mansoni Population In The Western Region Of Saudi Arabia. Tropical Biomedicine 28:
90–101
17. Sire C, Durand P, Pointier J-P, Theron A (2001). Genetic Diversity Of Schistosoma
mansoni Within And Among Individual Hosts (Rattus Rattus): Infrapopulation
Differentiation At Microspatial Scale. International Journal For Parasitology 31:
1609–1616
18. Stothard, J. R., Hughes, S., & Rollinson, D. (1996). Variation Within The Internal
Transcribed Spacer (Its) Of Ribosomal Dna Genes Of Intermediate Snail Hosts Within
The Genus Bulinus (Gastropoda: Planorbidae). Acta Tropica, 61(1):19-29
19. Tsai, M.-H., Marx, K. A., Ismail, M. M., & Tao, L.-F. (2000). Randomly Amplified
Polymorphic Dna (Rapd) Polymerase Chain Reaction Assay For Identification Of
Schistosoma mansoni Strains Sensitive Or Tolerant To Anti-Schistosomal Drugs. Journal
Of Parasitology, 86(1): 146-149.
20. Oliveira A, Silva D, Zanotti-Magalhaes E, Abdel-Hamid A, Ribeiro-Paes J (2008).
Schistosome/Mollusk: Genetic Compatibility. Genet. Mol. Res. 7: 518-526
21. Spada Rgm, Silva D, Abdel-Hamid A-Z , Sobral-Hamaguchi Ss, Zuim Nrb, Zanotti-
Magalhães Em, Magalhães La, Ribeiro-Paes Jt (2002). Genetic Markers Between
Biomphalaria glabrata Snails Susceptible And Resistant To Schistosoma mansoni Infection.
Mem Inst Oswaldo Cruz 97: 53-58
22. Barral, V., This P, Imbert-Establet D., Combes C., & Delseny M. (1993). Genetic
Variability And Evolution Of The Schistosoma Genome Analysed By Using Random
Amplified Polimorphic Dna Markers. Mol Biochem Parasitol., 59(2):211-21
Publicado
2018-09-08
Como Citar
1.
Mendes T, Calado M, Clemente I, Belo S, Afonso A. Análise genética de estirpes de Schistosoma mansoni sensíveis e resistentes a praziquantel por rapd-pcr. ihmt [Internet]. 8Set.2018 [citado 5Nov.2024];12:41-5. Available from: https://anaisihmt.com/index.php/ihmt/article/view/189
Secção
Artigos Originais
