Alimentação artificial de Rhipicephalus microplus: Avaliação do efeito de anticorpos policlonais

  • Sandra Antunes Estudante de doutoramento em Ciências Biomédica, Ramo de Parasitologia. UEIPM. Instituto de Higiene e Medicina Tropical, Centro de Malária e Outras Doenças Tropicais
  • Joana Lérias Bolseira de Investigação. CIISA, Faculdade Medicina. Veterinária., Universidade Técnica Lisboa
  • Octávio Merino Early Stage Researcher financiado por POSTICK ITN (Post-graduate training network for capacity building to control ticks and tick-borne diseases). Instituto de Investigación en Recursos Cinegéticos, Universidad Castilla-La Mancha, Ronda de Toledo, Ciudad Real, Espanha
  • Virgílio do Rosário Professor Catedrático jubilado. UEIPM. Instituto de Higiene e Medicina Tropical, Centro de Malária e Outras Doenças Tropicais
  • José de la Fuente Professor e investigador no Instituto de Investigación en Recursos Cinegéticos, Universidad Castilla-La Mancha, Ronda de Toledo, Ciudad Real, Espanha e no Department of Veterinary Pathobiology, Center for Veterinary Health Sciences,Oklahoma State University, Stillwater, OK 74078, USA.
  • Ana Domingos Investigadora auxiliar com agregação.UEIPM. Instituto de Higiene e Medicina Tropical, Centro de Malária e Outras Doenças Tropicais
Palavras-chave: Vector, anticorpos policlonais, alimentação artificial, Babesiose, Boophilus

Resumo

As carraças são ectoparasitas hematófagos obrigatórios que transmitem uma grande variedade de agentes patogénicos sendo considerados os principais vectores de doenças no caso de animais. Os piroplasmas Babesia bovis e B. bigemina são transmitidos essencialmente por carraças Rhipicephalus microplus e R. annulatus, consideradas os ectoparasitas de maior impacto económico na produção de gado. O método mais utilizado no controlo destes vectores continua a ser o uso de acaridas apesar das desvantagens que apresentam. As vacinas contra carraças não têm tido o impacto esperado devido à dificuldade em identificar antigénios eficazes. O antigénio calreticulina foi identificado em R. annulatus num estudo anterior onde foi demonstrado o seu envolvimento no processo de infecção por B. bigemina. Para testar este antigénio em ensaios de vacinação, a sua caracterização prévia é essencial. Uma alternativa de baixo custo para testar antigénios é a observação dos efeitos biológicos causados pela inoculação na carraça de drogas ou anticorpos contra uma determinada proteína. A alimentação artificial de carraças, apesar de não permitir a substituição de ensaios em gado, possibilita a obtenção de dados críticos na selecção de antigénios optimizando recursos. No estudo aqui apresentado pretendemos avaliar o efeito de anticorpos policlonais anti-antigénios de interesse no processo de alimentação e infecção, em carraças fêmea, R. microplus, alimentadas artificialmente.

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Publicado
2018-09-08