O impacto do efluxo na suscetibilidade reduzida a biocidas e resistência a fluoroquinolonas em Staphylococcus pseudintermedius

Marta Leal; Constança Pomba; Catarina Morais; Sofia Santos Costa; Bárbara Ramos; Isabel Couto; Patrícia Abrantes

doi:10.25761/anaisihmt.460

Marta Leal Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Portugal
Constança Pomba CIISA, Centre of Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterinária, Universidade de Lisboa, Portugal; GeneVet, Laboratório de Diagnóstico Molecular Veterinário, Carnaxide, Portugal
Catarina Morais Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Portugal
Sofia Santos Costa Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Portugal
Bárbara Ramos Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Portugal
Isabel Couto Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Portugal
Patrícia Abrantes Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Portugal

DOI: https://doi.org/10.25761/anaisihmt.460

Palavras-chave: Staphylococcus pseudintermedius, efluxo, biocidas, fluoroquinolonas, resistência, animais de companhia

Resumo

Staphylococcus pseudintermedius é o principal agente bacteriano de infeções de pele e tecidos moles (SSTI) em animais de companhia. O escalar da resistência aos compostos antimicrobianos, associada a fenótipos de multirresistência, coloca em risco a terapêutica a aplicar no controlo de infeções de pele causadas por este agente patogénico. O efluxo como mecanismo de resistência tem sido negligenciado para esta espécie. O objetivo deste estudo consistiu em avaliar a contribuição do efluxo para a suscetibilidade reduzida a biocidas e fluoroquinolonas. A estimativa de valores de cut-off (COWT) permitiu identificar uma população de S. pseudintermedius com suscetibilidade reduzida ao biocida brometo de tetrafenilfosfónio (TPP). A caracterização da atividade de efluxo foi avaliada por dois métodos: determinação de concentrações mínimas inibitórias (CMI) na presença de inibidores de efluxo e ensaios de acumulação/efluxo de brometo de etídeo por fluorometria em tempo real. A utilização destas duas metodologias permitiu associar este fenótipo de suscetibilidade reduzida a um aumento da atividade de efluxo. Relativamente à fluoroquinolona ciprofloxacina, a resistência é mediada primariamente pela ocorrência de mutações nos genes alvo deste antibiótico, o que pode mascarar a contribuição do efluxo neste fenótipo. Este estudo evidencia a relevância da resistência mediada por efluxo em estirpes clínicas de S. pseudintermedius, em particular a biocidas, o que pode comprometer a terapêutica a aplicar no controlo de infeções da pele e tecidos moles em animais de companhia.

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